Micropropagation of pongamia pinnata pdf

With the increasing production of oil for biofuel, large amounts of oil cake are available for livestock feeding. The database and code is licensed under a creative commons attributionnoncommercialsharealike 3. Oct 16, 2007 a complete protocol is presented for the first time for the micropropagation of pongamia pinnata, a biofuel tree, using cotyledonary nodes derived from axenic seedlings multiple shoots were induced in vitro from nodal segments through forced axillary branchi. The wood is yellowish white, coarse, hard, and beautifully grained, but not durable.

Micropropagation is the practice of rapidly multiplying stock plant material to produce many progeny plants, using modern plant tissue culture methods micropropagation is used to multiply plants such as those that have been genetically modified or bred through conventional plant breeding methods. Sprouting of buds was 64% on medium devoid of plant growth regulators pgr. Found in east asia, pongamia pinnata or pongnam is a tropical, fastgrowing, medium to large tree usually growing about 1525 m in height and 5080cm in bole diameter. Feasibility study on thermal cracking of pongamia pinnata. Pongamia pinnata is a fastgrowing, mediumsized, evergreen or briefly deciduous, glabrous shrub or tree with a broad crown of spreading or drooping branches. It is one of the few nfixing trees that produce oilseeds sangwan et al. Karanja is an important ayurvedic medicine, used predominantly in skin diseases. The fresh bark of pongamia pinnata is sweet and mucilaginous to taste, soon become bitter and acrid. Micropropagation is the truetotype propagation of a selected genotype using in vitro culture techniques. The trunk is generally short with thick branches spreading into a dense hemispherical crown of dark green leaves.

Which has been found to consist of five sugars viz dmannose, dglucose, dxylose, raffinose, lfructose along with five amino acids, alanine, phenylalanine, tyrosine acid, tryptophane, and threonine. Effects of ethanolic extract of pongamia pinnata linn. Pongamia pinnata has generated a lot of scientific interest chapter 1 not only as a source of raw material for biodiesel, but also for its industrial and medicinal uses. Herbal practices similar to the ones given below may also have been received from other people from same different parts of the country. This multipurpose tree species is well adapted for various agro climatic conditions and is one of the most extensively chemically investigated plants. Pongamia pinnata is a very fastgrowing medium size plant with an average height of 3040 feet and spreads canopy for casting moderate shade orwa et al. Pongamia millettia pinnata syn ponamia pinnata summary pongamia millettia pinnata, formerly known as pongamia pinnata, is a treeshrub with a broad distribution from india, through central and southeastern asia, indonesia and.

Millettia pinnata, also known as pongamia pinnata or pongamia glabra, is common throughout asia and thus has many different names in different languages, many of which have come to be used in english to describe the seed oil derived from m. We present three process models for production of aviationfuel from microalgae, pongamia pinnata seeds and sugarcane molasses. Pongamia pohngahmeah for most, pohngaymeeah for florida folks is a semideciduous, nitrogenfixing legume tree that can be grown in diverse tropical and subtropical marginal lands. Millettia pinnata is a species of tree in the pea family, fabaceae, native to eastern and tropical asia, australia and pacific islands. Pdf micropropagation of mature pongamia pinnata pierre. Read this article to learn about the techniques, factors, applications and disadvantages of micro propagation process and also about production of diseasefree plants and its merits and demerits. Analgesic activity of ethanolic extract of pongamia. We have used axillary meristem as explants for micropropagation of p. Propagation techniques, evaluation and improvement of the. Pongamia pinnata is a native to many countries including india, malaysia, indonesia, taiwan, bangladesh, sri lanka and myanmar. Pongamia biomass producer bioenergy information for. The study was undertaken with an objective to develop a protocol for micropropagation of pongamia pinnata pierre through shoot apex segments shoot of 0.

Detail study on the properties of pongamia pinnata karanja for the production of biofuel bobade s. Vent, an oil producing tree legume, popularly known as karanja, is the most widely available nonedible oil tree in india. Axillary buds, biodiesel plant, leaf, micropropagation, pongamia, plant growth regulators, plant tissue culture. Pongamia pinnata various types of soil like alkaline, salty, sandy, clay, stony and waterlogged soils and also it. In our country there are more than 300 species of trees, which produce oil bearing trees. Sprouting of the central meristem to form a single shoot. It has a broad crown of drooping branches, straight or crooked bole, extensive lateral roots, and creamy white or pink flowers that form into clusters. Incorporation of ba, kn, or z was ineffective in enhancing sprouting frequency or. The collection and extraction of oil is carried out by indian biodiesel corporation, baramati. It is often known by the synonym pongamia pinnata as it was moved to the genus millettia only recently. Multiple shoots were induced in vitro from nodal segments through forced.

Policy makers, scientists as well as farmers have turned their attention to this species with great gusto since the benefits to be derived affect all the stakeholders. Technoeconomic analysis of renewable aviation fuel from. Pongamia pinnata has recently been recognized as a viable source of seedoil for production of biofuel to meet the demand of the burgeoning. Hence this paper aims in finding out feasibility of using pongamia pinnata oil to ci engines directly by catalytic cracking instead of going for transesterificaiton. Pongamia indian beech is a genus having one species pongamia pinnata l. Bioprocess optimization for biodiesel production from pongamia pinnata. But the best spacing for the commercial cultivations would be 5 mtr x 5 mtr to have 400 tress per hectare. Pongamia pinnata is a deciduous, salt tolerant, drought resistant nitrogen fixing leguminous tree and their seeds are well known for extraction of nonedible oil. Micropropagation of mature pongamia pinnata pierre. Multiple shoots were induced in vitro from nodal segments through forced axillary branching. In the present study, the crude oil extracted from pongamia pinnata seed was used to synthesize biodiesel fatty acid methyl esters by tranesterification with. For this there is need to develop pongamia reproducible in vitro regeneration. Karanja, millettia pinnata is a multipurpose tree which is valued for its oil and also supplies dyestuff, fuel, wood, medicines and various other commodities.

Citeseerx micropropagation of karanj pongamia pinnata. Pongamia pinnata linn pierre commonly known as karanj contains various phytoconstituents belonging to alkaloids, glycosides, flavonoids, fixed oils and carbohydrates. Pongamia biodiesel pongamia biodiesel project services. Pierre has been receiving considerable attention since its role as a feed stock for biodiesel production was defined and confirmed. Abstract pongamia pinnata is a legume plant which has great. Detail study on the properties of pongamia pinnata karanja. The present paper deals with chemical examination of defatted seeds of the plant pongamia pinnata l. Determination of in vitro sunscreen activity of pongamia. Petroleum ether, chloroform, methanol, and water extracts of p. Direct adventitious shoot bud formation on hypocotyls. The bark on the main trunk is grey or greyishbrown in colour and relatively smooth. Department of biotechnology, gulbarga university, gulbarga585106, karnataka, india corresponding author email. Derris indica is a nitrogenfixing, wild, perennial, leguminous tree native to humid and subtropical environments occurring widely along the coasts and riverbanks of india and myanmar. Molecular analysis using rapd and issr of micropropagated plants of p.

Pongamia pinnata may be cultivated in the waste land, or in any other chosen land as per the parameters given earlier in the presentation. We bioenergy plantations australia are a company dedicated to the creation of an economically and environmentally viable alternative to fossil fuels by the use of pongamia pinnata aka milletia pinnata. Technoeconomic analysis of renewable aviation fuels has not been widely considered, despite the increasing global attention that the field has received. Chemical examination of the seeds of pongamia pinnata l. High frequency direct organogenesis and evaluation of genetic. Useful tropical plants database 2014 by ken fern, web interface by ajna fern with help from richard morris. Micropropagation of pongamia pinnata through enhanced. In vitro screening of antilice activity of pongamia. List of various diseases cured by kalanchoe pinnata. Pongamia oil is derived from the seeds of the millettia pinnata tree, which is native to tropical and temperate asia. Research article physico chemical analysis of pongamia pinnata. High frequency direct organogenesis and evaluation of. Shrivastava v, kant t 2010 micropropagation of pongamia pinnata l. Pierrea native indian biodiesel tree from cotyledonary node.

Jain 1, shashi alok 1, deepak kailasiya 1, vinod kr. Evaluation of antibacterial activity of pongamia pinnata l. Index terms pongamia pinnata, cracking, tga, dsc, non edible oil, c. Use of the wood is limited to cabinetmaking, cartwheels. Pdf murashige and skoogs ms basal medium with benzylaminopurine ba, kinetin kn, zeatin z, and thidiazuron tdz were tested for induction of. Abstract pongamia pinnata is a legume plant which has great potential to be used as a biofuel feedstock.

Pierre a native indian biodiesel tree from cotyledonary node. Pongamia pinnata is a fastgrowing, mediumsized, evergreen or briefly deciduous, glabrous shrub or tree with a broad crown of spreading or drooping branches 303 title. Karanja pongamia pinnata benefits, usage, ayurveda details. Plant tissue culture technique has been used extensively to study the effect of. In this work reaction parameters namely 1 temperature, 2 time, 3 naoh concentration and 4. Employing plant tissue culture techniques for micropropagation and further plant improvement of p. This stage begins with the excision of meristem tissue from an identified stock plant. General physical properties of pongamia pinnata oil blends b10, b20 and diesel parameters pongamia pinnata diesel b20b10 ph 3.

At cjp we have a team of experts to guide the pongamia pinnata investors for developing a sustainable pongamia pinnata project who have a have a good understanding of the conditions on the ground, in terms of agronomic practices, improved cultivar development, water requirements and labor requirements. Uses, benefits, cures, side effects, nutrients in kalanchoe pinnata. Trending things to know about sanitizing and disinfecting your house. It is a species of the legume family and grows large pods that are rich in triglyceride oils that may be converted into biodiesel and aviation fuel. Regeneration of pongamia through tissue culture from immature cotyledons a through. Pierrea native indian biodiesel tree from cotyledonary node article pdf available january 2010 with 740 reads. From literature, it is apparent that there is a lot of scope for genetic modification of. Effect of c y tokinin and aux in on shoot proliferation from cot yledonary nodes of pongamia pinnata grown on ms medium after 4 week. Pongamia pinnata often called pongam tree, indian beech tree, millettia pinnata, honge tree is actually a midsized tree with countless characteristics. Your use of this pdf, the bioone complete website, and all posted and associated. Pierre is a medium sized glabrous tree popularly known as karanja in hindi, indian beech in english and pongam in tamil4. The roots of pongamia pinnata are good for cleaning foul ulcers, cleaning teeth, strengthening gums and gonorrhoea. Bioprocess optimization for biodiesel production from.

Panigrahi is a fastgrowing, multipurpose tree of the humid tropic. A complete protocol is presented for the first time for the micropropagation of pongamia pinnata, a biofuel tree, using cotyledonary nodes derived from axenic seedlings. Pappilonaceae is a nonedible oil of indian origin lakshmikanthan, 1978. Michael kane environmental horticulture department university of florida rapid clonal in vitro propagation of plants from cells, tissues or organs cultured aseptically on defined media contained in culture vessels maintained under controlled conditions of light and temperature micropropagation in vitro propagation. Aug 18, 2007 murashige and skoogs ms basal medium with benzylaminopurine ba, kinetin kn, zeatin z, and thidiazuron tdz were tested for induction of multiple shoots from maturetreederived axillary meristems of pongamia pinnata. Pongamia millettia pinnata syn ponamia pinnata summary pongamia millettia pinnata, formerly known as pongamia pinnata, is a treeshrub with a broad distribution from india, through central and southeastern asia, indonesia and into northern australia. The root paste is used for local application in scrofulous enlargement. Pongamia pinnata ethanolic extract ppee have shown the more significant p pongamia pinnata. Pierre is a bioenergy tree legume with the potential for high oil seed production and the added benefit of the ability to grow on marginal land scott et al. Citeseerx document details isaac councill, lee giles, pradeep teregowda. Multiple shoot bud induction and plant regeneration. Bank, tal baramati, dist pune, ms, india 2shardabai pawar mahila college, shardanagar, tel.

Biodiesel production from pongamia pinnata oil using. All the extracts were dissolved in distilled water to obtain 3 different concentrations 5%, 10%, and 20%. Uses, side effects, research karanja is an important ayurvedic medicine, used predominantly in skin diseases. Pongamia pinnata is a legume plant which has great potential to be used as a biofuel feedstock. Spacing differs from the soil conditions and terrain. Micropropagation of karanj pongamia pinnata pierre through shoot apex segmentsa medicinal and biofuel plant. Uses the pongam tree is cultivated for two purposes. It is found mainly in the native western ghats in india, northern australia, fiji and in some regions of eastern asia. Madhavarao buddepu, et al determination of in vitro sunscreen activity of pongamia pinnata l.

Micropropagation of pongamia pinnata through enhanced axillary branching 411 table 2. Lucknow, who introduced us to plant tissue culture and to the intricacies of the technique. Nov 28, 2017 new pongamia farmers, have your elevator pitch ready even most fellow farmers have never heard of the crop. Indian journal of natural products and resources vol. Which has been found to consist of five sugars viz dmannose, dglucose, dxylose, raffinose, lfructose along with five. Kalanchoe pinnata herb uses, benefits, cures, side effects. Incorporation of ba, kn, or z was ineffective in enhancing sprouting frequency or induction. The plant extract contains flavonoids, carbohydrates, glycosides, international journal of chemtech. Pdf micropropagation of pongamia pinnata through enhanced. Effect of gibberellic acid on elongation of shoots concentration of growth regulator um mean number of shootsnode mean shoot length mm. Pongamia pinnata pongam, karum tree, poongaoil tree.

Micropropagation of mature pongamia pinnata pierre bioone. Micropropagation of karanj pongamia pinnata pierre. Currently, the existing stands of karanja in india are naturally grown. Multiple shoot bud induction and plant regeneration studies of. Pongamia pinnata oil with conventional diesel with b10 and b20 proportions are furnished in the table3. The traditional knowledge examples given below are just an indicativeillustrative sample. Pair of meristematic masses mm developed from central meristem c.

Research article physico chemical analysis of pongamia. Karanja twigs were used as tooth brush in ancient times. How kalanchoe pinnata is effective for various diseases is listed in repertory format. Efficacy of two dominant marker systems, issr and teaflp for assessment of genetic diversity in biodiesel species pongamia pinnata. Most of the tamil nadu physicians of indian system of traditional medicine ayurveda and siddha use pongamia pinnata to treat various kinds of diseases including diabetes mellitus5.

Detail study on the properties of pongamia pinnata. In vitro micropropagation of biofuel feedstock pongam pongamia. Preparation of biodiesel from crude oil of pongamia pinnata. August, 2011 drug invention today, 2011, 38, 197199 199 this sunscreen property of the pongamol compound may be attributed to the. Pongamia pinnata leguminoceae, subfamily papilion oideae is a medium sized tree that about 7 8 meters high with a trunk diameter of more than 50 cm. Evaluation of efficiency of propagation techniques tree. Micropropagation of pongamia pinnata a sustainable biofuel plant species for semi arid climatic conditions vinod kumar patil and naik, g.

A simple and improved method for isolation of karanjin. These non edible oil seeds are also used for lightning purpose at night. Transesterification of pongamia pinnata oil using base. Multiple shoot bud induction and plant regeneration studies. The pongamia millettia pinnata plant is a leguminous tree that is found through much of asia, northern australia and the pacific islands. Pdf micropropagation of karanj pongamia pinnata pierre. Plants can be propagated by sexual through generation of seeds or asexual through multiplication of vegetative parts means.

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